우식원세균 바이오필름 모델에서 다양한 농도의 Galla Chinensis 추출물의 항균효과
Antibacterial effect of different concentrations of Galla Chinensis extract on cariogenic bacteria in a biofilm model
김은정, 진보형,
소속 상세정보
김은정 ( Kim Eun-Jeong ) - 서울대학교 치의학대학원 치의학연구소
진보형 ( Jin Bo-Hyoung ) - 서울대학교 치의학대학원 예방치학교실
Abstract
Objectives: Galla Chinensis inhibited the adherence of planktonic oral bacteria and acid production by cariogenic bacteria. However, little is known about the relevant conditions of Galla Chinensis extract (GCE) exposure time and concentration and the effect of GCE on the structural and functional activity of cariogenic bacteria. The antibacterial effects of natural G. Chinensis extract on S. mutans, S. sanguinis, and S. oralis biofilms were evaluated in vitro.
Methods: Biofilms formed on glass surfaces were treated with different concentrations of GCE at different exposure times. The effects were assessed by examining the bactericidal activity, acidogenesis, minimum inhibitory concentration, and morphology.
Results: There was a statistically significant difference in the bacterial growth inhibition depending on the concentration of the GCE, with bacterial growth being inhibited as the concentration of GCE increased. A concentration of 1.0 mg/ml GCE had similar bactericidal effects against S. mutans and S. oralis biofilms to those produced by 2.0 mg/ml CHX. In the 1.0 mg/ml GCE group, incomplete septa were also observed in the outline of the cell wall, together with disruption of the cell membrane. In addition, there was also a slight exudation of the intracellular content from the bacteria in the 1.0 mg/ml GCE and 2 mg/ml CHX groups.
Conclusions: These results indicate that GCE inhibits the growth of S. mutans, S. sanguinis, and S. oralis with increasing concentrations. It alters the microstructure of S. mutans biofilms. These results suggest that GCE might be a useful anti-bacterial agent for preventing dental caries.
키워드
Antibacterial effects; Biofilms; Galla Chinensis; Streptococcus mutans
원문 및 링크아웃 정보
등재저널 정보